WebThe CuAAC Biomolecule Reaction Buffer Kit (THPTA based) provides sufficient amounts to perform 25 CuAAC experiments à 200 μl using 2 mM CuSO 4, 10 mM THPTA and 100 mM Na-Ascorbate in 100 mM Na-Phosphate reaction buffer. A general protocol for labeling of biomolecules (see 3.) is outlined below. WebThe Click-iT Protein Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two moieties together. Each kit includes sufficient material to perform 25 reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.
Protein Click Reaction Buffer Kit - Kerafast
WebClick Chemistry Protein Click Reaction Buffer Kit provides all the necessary reagents to perform an efficient copper-catalyzed click reaction with any azide or alkyne … WebMar 9, 2015 · 1.4 Prepare 40 mL of 1X Click-iT® HPG reaction buffer by transferring all of the solution (4 mL) in the Component C bottle to 36 mL of deionized water. Rinse the Component C bottle with some of the diluted Click-iT® HPG Reaction buffer to ensure the transfer of all of the 10X concentrate. rowecord holdings ltd
Click Chemistry Reaction Buffer Kits Click Chemistry Tools
WebThe Click-iT® reaction buffer additive is susceptible to oxidation and is the limiting factor to the Click-iT® reaction cocktail’s effectiveness over time. Table 3. Click-iT® reaction cocktails. Reaction components Number of plates 0.5 1 2 Click-iT® reaction buffer (Component C) 2.14 mL 4.28 mL 8.56 mL CuSO 4 (Component D) 100 µL 200 µL ... WebRinse the Component E bottle with some of the diluted Click-iT® EdU reaction buffer to ensure the transfer of all of the 10X concentrate. To make smaller amounts of the Click-iT® reaction buffer, dilute volumes from the Component E bottle 1:10 with deionized water. After use, store any remaining stock solution at 4˚C or at room temperature. WebNov 6, 2024 · In vitro OGT reaction (100 μl) contained 10 μM TAB1 (7–409 construct), 50 nM full-length human OGT in TBS buffer, pH 7.5, with 0.1 mg ml −1 BSA. The reaction was initiated by addition of UDP ... streaming rust