Pshuttle-gfp
WebNov 2, 2015 · The Ad vector pShuttle-CMV (Stratagene) was rendered replication defective by deletion of the E1 and E 3 genes [ 23 ]. The GFP coding sequence was amplified from the pEGFP-N1 vector (Clontech) using the primer pair 5′-ATGGTGAGCAAGGGCGAG-3′ and 5′-TTACTTGTACAGCTCGTCC-3′. WebJan 7, 2024 · Product Name : pShuttle article : bacterial resistance : Kanamycin cloning : growth notes : Vector pShuttle is used for expression of transgenes under a chosen (non …
Pshuttle-gfp
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WebReviews. Product Specs. Item pShuttle-IRES-hrGFP-1. Company Agilent Technologies. Price Supplier Page View Company Product Page. Catalog Number 240081. Host mammalian. … WebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ control pLVX …
WebDec 1, 2009 · The formed plasmids, pShuttle-GFP-TPE and pShuttle-GFP-D19K, respectively, were the final shuttles. These two plasmids were recombined with pAdEasy-1 to generate … WebMethods: The pSport 1-mSD274 plasmid containing the full-length PD-L1 cDNA of the mouse was digested and subcloned to the shuttle plasmid pShuttle-GFP-CMV (-), and then the adenovirus skeleton plasmid pAdxsi-GFP-CMV-PD-L1 was constructed by enzymolysis and ligation, transformed into DH5α sensitive bacteria, and screened for positive clones.
Webwith green fluorescence protein (GFP) gene. The PDX1 gene from pShuttle-GFP-CMV-PDX1 was transferred into adenoviral vector (pAdxsi) (Gibco-Invitrogen) and linearized with I-CeuI + I-SceI enzyme. All sequences were confirmed by DNA sequencing. The confirmed recombinant adenoviral Differentiation of PDX1 gene-modified human umbilical cord Web(2) Cathepsin B Improves ß-Amyloidosis and Learning and Memory in Models of Alzheimer’s Disease Journal of Neuroimmune Pharmacology Adenoviral Vector System (#240010), pShuttle-IRES-hrGFP-1 vector (#240081) and pShuttleIRES-hrGFP-2 vector (#240082, all from Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s …
WebMay 30, 2024 · We constructed three new adenoviral vectors based on the shuttle plasmid pShuttle (Stratagene, La Jolla, CA, USA). The vectors (pAd shRNA/mU6, pAd shRNA/H1, and pAd shRNA/U6) all contain an shRNA expression cassette and an enhanced GFP (EGFP) marker, and all three constructs have the same cloning sites . The shRNA cassette is …
WebJul 24, 2007 · TrkA-PC12 cells were transfected with a cDNA for GFP-tagged Rab7 (Rab7-GFP) and Pincher-HA. In these cells, P-Trk and Rab7-GFP were rarely colocalized in cytoplasmic structures (weighted colocalization coefficient for Rab7-GFP = 0.16 ± 0.14; P-TrkA = 0.11 ± 0.19; n = 17), even after 1 h of NGF treatment ( Fig. 4 C Upper ). gabby\u0027s banquet and event facilityWebtor pShuttle-CMV-EGFP. The plasmid pShuttle-CMV-EGFP was linearized with EcoRV and Bg1II, and was afterward dephosphorylated with alkaline phosphatase (CIAP), which was then ligated to a rHSG gene segment by T4 DNA ligase. The linearized pShuttle-GFP-rHSG was transformed into the competent BJ5183 strain which was transformed with pAdxsi. gabby\u0027s barber shop chicagoWebMethods: From pcDNA3.1-NELL1, NELL1 gene sequence was obtained, then NELL1 gene was subcloned into pShuttle-GFP-CMV (-)TEMP vector which was subsequently digested with enzyme and inserted into pAdxsi vector to package the recombinant adenovirus vector (pAdxsi-GFP-NELL1). gabby\u0027s bbq heightsWebMethods: hVEGF165 was amplified via RT-PCR before recombinant with pShuttle- green fluorescence protein;green fluorescent protein (GFP)-CMV. Then the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine (TM) 2000 for packaging and amplifying. hVEGF165 mRNA expression in BMSCs cells was tested. gabby\u0027s bbq houstonWebRecombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or … gabby\u0027s bbq houston locationsWebpShuttle pShuttle CMV pRL CMV pCMV-HA pVAX1 pVAST pCI pCI neo+ pCMV-myc pflag-CMV2 pcDNA3.1 + pcDNA3.1 zeo+ pcDNA3.1 neo+ pcDNA3.1 MycHisA pcDNA3.1 MycHisB pcDNA3.1-HBV genome pcDNA3-mRFP Hsp70-pcDNA3.1 pcDNA 4 Max B psuper GFP neo psuper Retro GFP neo pSilencer4.1CMV puro+ pSilencer4.1CM neo+ psuper GFP neo … gabby\u0027s beauty supply baldwinWebpShuttle-IRES-hrGFP-1 Vector by Agilent Technologies Manufacturer Agilent Technologies Model: 240081 Be the first to review this product Recombinant adenoviruses are a versatile tool for gene delivery and expression. gabby\u0027s bbq houston texas