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Pslf172

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WebELLULAR DNA replication requires precise regu- lation to ensure that the genome is replicated once completely in each cell cycle, and that the process of replication is coordinated with proper cell cycle entry and subsequent nuclear division. WebDec 30, 2005 · This restriction product was ligated into Bgl II/ Sal I-digested pSLF172 together with Nco I/ Xho I digested PCR product generated with oligonucleotides A and C (see above). The pnmt-GFP and pcpy-GFP vectors have been described elsewhere [25]. Protein extraction and Western analysis.

The fission yeast homologue of Orc4p binds to replication origin …

WebJun 10, 2005 · The synthesis of O-mannosyl glycans is initiated in the endoplasmic reticulum (ER) by the transfer of a mannosyl residue from dolichyl phosphate-activated mannose (Dol-P-Man) to specific serine/threonine residues of proteins entering the secretory pathway ( Strahl-Bolsinger et al ., 1999; Willer et al ., 2003 ). Webepitope in its C-terminal region. To construct pSLF172(L)-pka1, the pka1 gene from pSLF372(L)-pka1 was digested with BglII and NotI. The digested fragment was cloned … hurlingham park brighton

pSLF272 - 87610 ATCC

Webresultant fragments were cloned into the PstI and BglII sites of pSLF172 (63), which was designed to add a 3xHA tag at the C terminus of the respective protein. Since the plasmids obtained (pSLF172-PKC1, pSLF172-PKC1T1125A, and pSLF172-PKC1S1143A) lacked the terminator region WebOct 1, 2024 · 4 beds, 2 baths, 1624 sq. ft. house located at 172 Longfellow Dr, Palm Springs, FL 33461 sold for $370,000 on Oct 1, 2024. MLS# RX-10646249. Modern, sleek, European … WebMar 19, 2010 · (Stellar MLS) 5 beds, 3.5 baths, 3901 sq. ft. house located at 8172 Narrow Leaf Pt, SANFORD, FL 32771 sold for $500,000 on Mar 19, 2010. MLS# O4915354. * * … hurlingham polo clothing

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The fission yeast homologue of Orc4p binds to replication …

WebTo construct pSLF172 (L)-pka1 plasmid, the pka1 gene from the plasmid pSLF372(L)-pka1 was digested with the restriction enzymes BglII and NotI. The digested fragment was … WebDec 22, 2000 · A NotI linkered genomic MPK4 fragment including the 1150 bp promoter was amplified from La-0 genomic DNA and cloned into that site of pSLF172 (Forsburg and Sherman 1997) to produce a C terminally triple HA-tagged MPK4. The activation loop mutant (T201A/Y203F) was made using the QuickChange kit (Stratagene). mary fullertonWebMolecular Biology of the Cell Vol. 13, 1132–1143, April 2002 The Schizosaccharomyces pombe Aurora–related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation hurlingham polo in the park

"WebPoint mutations in pSLF172-hsk1 were generated using QuickChange PCR (Stratagene). Transformants were isolated in the presence of 5 μg/ml thiamine, and expression was … " - Pslf172

Pslf172

Vector Table > PombeNet Forsburg Lab > USC Dana and …

WebMay 10, 2002 · The plasmid pRCE81X was derived from the plasmid pREP81X (30) by replacing the 2.2-kb nmt1 promoter-terminator DNA fragment with thenmt1 promoter-terminator DNA fragment of pSLF172 (31). Theorc4 + gene was synthesized by PCR and inserted into the XhoI/NotI sites of pRCE81X, yielding pRCE81X-orc4. Webfull strength nmt expression vectors allowing 8XHis HA double tag fusion at C-terminus (1072) or N-terminus (1073). Compatible with pSLF172/173 series. (Forsburg lab) …

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Webof pSLF172 (38). The orp41 gene was synthesized by PCR and inserted into the NotI site of pRCE3X. Strain YRC4 (h2 ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 … WebMar 17, 2014 · 2003). 3HA tag was fused in frame to the C‐terminus of fission yeast Rec8 protein using pSLF172 (Forsburg & Sherman 1997). All strains used in this study are listed in Table S3 (Supporting Information). Synchronous meiotic cultures of budding and fission yeast and monitoring meiotic progression were conducted as described previously (Ohta …

WebJun 1, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2+ ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 promoter in the vector pSLF172 ( 14 ). The strain expressing HA-tagged Cdc13, GBY248, was constructed in a similar manner. WebOct 13, 2024 · Thecdc19-M4 mutant was constructed and cloned into the HA-tagging nmt1 + expression vector pSLF172 (Forsburg and Sherman, 1997) as described previously (Forsburg et al., 1997). Plasmid pMF56, expressing N-terminally HA-tagged Cdc18p under control of the nmt1 + promoter was a kind gift of Marco Muzi-Falconi and Tom Kelly (Muzi …

WebMar 16, 1999 · The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. Although ORC appears to play... The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces WebThe S. pombe expression vector pREP3X, pSLF172, and pSLF173 series were obtained from recombinant DNA vec-tors in the American Type Culture Collection (ATCC) (Table 1). To construct fluorescent protein-tagged vectors, the coding sequences of the fluorescent proteins were cloned into the S. pombe autonomously replicating sequence (ARS) element-

WebJun 10, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2 + ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 …

Webof pSLF172 (38). The orp4+ gene was synthesized by PCR and inserted into the Notl site of pRCE3X. Strain YRC4 (h-ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 protein (amino acids 1-486), and strain YRC5 (h-ura4-D18pRCE3X-orp4C), expressing the C-terminal terminal half of Orp4 protein (amino acids 436-972), were constructed mary fullerton obituaryWebpSLF172,272,372 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially … hurlingham primary schoolWebof pSLF172 (38). The orp41 gene was synthesized by PCR and inserted into the NotI site of pRCE3X. Strain YRC4 (h2 ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 protein (amino acids 1–486), and strain YRC5 (h2 ura4-D18 pRCE3X-orp4C), expressing the C-terminal terminal half of Orp4 protein (amino acids 436–972), were constructed hurlingham polo 1875 manchesterWebJun 3, 1997 · As shown in Fig. 2 A, an anti-Cdc19p antibody recognizes a low amount of endogenous Cdc19p protein in the control lysate, and substantially increased amounts in … hurlingham property for saleWebproducts were digested with XhoI and BamHI, cloned into pSLF172, and transformed into S. pombe 201402 by electroporation. Transformed cells with the ura phenotype were … hurlingham prepWebPlasmid pREP3‐adh: The nmt1‐MCS‐3xHA‐term cassette from pSLF172 (Forsburg and Sherman, 1997) was cloned as a PstI/SacI fragment into pREP3X (gift of S.L. Forsburg), giving rise to plasmid pREP3X‐tag. Subsequently a XhoI/NotI fragment from pREP3X‐tag was substituted with a hybridized tw75/tw76 primer adapter giving pREP3‐SX. hurlingham office park sandtonWebJul 4, 2024 · landed at Hartsfield-Jackson Intl - ATL. Saturday 04-Jul-2024 04:00PM CEST. (on time) Saturday 04-Jul-2024 07:20PM EDT. (15 minutes early) 9h 20m total travel time. … hurlingham quadrangle scottish country dance