2024 Tfeb-s211a

Tfeb-s211a

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Web17 Aug 2024 · In particular, TFEB S142A showed impaired phosphorylation of S142 and S138; TFEB S138A showed impaired phosphorylation of S138, but normal S142 phosphorylation; importantly, in TFEB S211A, which still retained the ability to be exported from the nucleus, S142 and S138 were normally phosphorylated (Fig. 4c). These data … Web12 Apr 2024 · PLX4720 ablated TFEB S142 phosphorylation in A375 cells (Fig. 3a ). An in vitro kinase assay using bacterially purified proteins showed that active ERK directly …

TFE3 and TFEB increase TMEM55B expression to cluster

Web24 Mar 2024 · On day two, levels of TFEB (S211A)-GFP and TFEB targets were down to Dark levels, however the AT8 + and AT180 + p-Tau levels seem to have gradually raised but still remained significantly lower than their starting levels . Taken together, for the first time, these results suggest that light-induced, optogenetic-based expression of TFEB can … WebTFEB-mediated endocytosis drives assembly of the MTORC1-containing nutrient sensing complex through the formation of endosomes that carry the associated proteins RRAGD, the amino acid transporter SLC38A9, and activate AKT/protein kinase B (AKT p-T308). does cool whip have egg in it

Oxidation of multiple MiT/TFE transcription factors links oxidative ...

Web1 Jul 2016 · The transcription factor EB (TFEB) plays a pivotal role in the regulation of basic cellular processes, such as lysosomal biogenesis and autophagy. The subcellular … Web1 Mar 2024 · Notably, dose-response studies demonstrated that even relatively low levels of TFEB S211A expression impair the proliferation of 32D.3 myeloid cells, as well as the … WebTranscription factor EB (TFEB), encoded by Tcfeb gene, was identified as a master transcription factor for lysosome biogenesis and autophagy ().TFEB expression is up … ezy watermark app

An FGF15/19-TFEB regulatory loop controls hepatic …

TFEB at a glance - PubMed

Web22 Mar 2024 · Transcription factor EB (TFEB) is a basic helix–loop–helix–leucine–zipper transcription factor, which is considered the main regulator of lysosomal biogenesis and autophagy, and mediates various physiological processes, including immune response, neurodegenerative diseases, and metabolic diseases ( 12 – 15 ). WebNational Center for Biotechnology Information does coop sell phone chargersWeb1 Aug 2024 · Furthermore, in HeLa WT cells, transfection of constitutively active TFEB S211A-mCherry (S211 non-phosphorylatable mutant) [30], or WT TFEB-mCherry, resulted in predominant nuclear localization of TFEB in the presence of 4-HNE (Fig. S2). Taken together, these results demonstrate that 4-HNE promotes autophagosome and lysosome … ezy-way.com.au

"Web1 Apr 2024 · To test whether Nanog was a potential target gene of TFEB, a constitutively active form of TFEB (TFEB-AA, mutated S142/S211 to S142A/S211A) and a reporter … " - Tfeb-s211a

Tfeb-s211a

Multistep regulation of TFEB by MTORC1. - Abstract - Europe PMC

Web15 Nov 2013 · Importantly, our analyses established that the induction of TFEB-S211A markedly impaired the proliferation and tumorigenic potential of Eµ-Myc lymphoma. Mechanistically, this proliferative arrest was associated with the induction of the senescence program, and with an increased extracellular acidification rate. Web5 Mar 2024 · To further test the role of TFEB in vivo, the authors performed mouse xenograft experiments using AML cells with dox-inducible TFEB S211A. Consistent with ex vivo …

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Web24 Nov 2024 · We demonstrate that TFEB is a direct substrate of PTEN phosphatase and PTEN deficiency increases exosome secretion in cholangiocarcinoma by reducing lysosome biogenesis, which further promotes tumor metastasis.

Web5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A nuclear localization. (A) Validation of the newly generated p-TFEB (S211) antibody. HeLa cells were transfected with the indicated MYC-tagged plasmids (WT, wild-type TFEB; … WebMYC-tagged plasmids (WT, wild-type TFEB; S211A TFEB mutant or EV, empty vector), lysed, and treated or not with lambda phosphatase (“λ”) before MYC immunoprecip-itation (IP) …

Total RNA was extracted from cellular lysates using the RNeasy Mini kit (Cat# 74104, Qiagen) according to the manufacturer’s instructions. cDNA was synthesized by reverse transcription of total RNA (500 ng per sample) using QuantiTect Reverse Transcription kit (Cat# 205311, Qiagen). The generated cDNA was … See more Cells were cultured in the following media: HeLa (ATCC) in MEM (Cat# ECB2071L, Euroclone); HEK293T (ATCC) in DMEM high glucose (Cat# ECM0728L, Euroclone); ARPE19 (ATCC) in … See more Reagents used in this study were obtained from the following sources: Antibodies to Phospho-p70 S6 Kinase (Thr389) (1A5) (Cat# 9206) (1:1000), … See more For experiments involving amino acid starvation, cell culture plates were rinsed twice with PBS and incubated in amino acid-free RPMI (Cat# R9010-01, USBiological) supplemented with 10% dialyzed FBS for 60 … See more The plasmid-encoding ΔNLS-TFEB-GFP was a kind gift of Shawn Ferguson (Yale School of Medicine, CT, USA). TFEB S138A-GFP was a kind gift of Chonglin Yang (Chinese … See more Web28 Jul 2024 · Flag-tagged S142A/S211A-TFEB mutant was cotransfected, in HEK293 cells, with a GFP-tagged TFEB construct (wild-type, S142A/S211A, or S142D/S211D). Co-immunoprecipitation analysis, using Flag antibody, …

WebThus, although CDK4 and CDK6 phosphorylate TFEB at multiple sites, the phosphorylation at Ser142 plays a major role in preventing TFEB from localizing to the nucleus. Supporting this notion, in vitro phosphorylation by CDK4 or CDK6 of TFEB(105–300) containing S142A, but not S211A, was strongly reduced compared with the WT .

Web11 May 2012 · S211 is a novel MTORC1-dependent phosphorylation site in TFEB required for the binding to YWHA. (A) Coomassie blue stained SDS-PAGE gel of co-immunopreciptated TFEB and YWHA from HeLa (CF7) cells treated with either DMSO or PP242. The asterisk (*) indicates IgG heavy and light chains. The arrowhead denotes actin. ezy way arncliffeWeb11 Feb 2013 · At stationary state, TFEB-S211A accumulates in the nucleus but also displays stable association with lysosomes ( Fig. 1, B and D; Martina et al., 2012 ). As expected, depletion of raptor did not affect the ability of TFEB-S211A to associate with lysosomal membranes ( Fig. 1, B and D ). does cool whip have lactoseWeb5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A … does coordinator in ny hospital use uniformWeb26 Jan 2016 · In addition, over-expression of TFEB-S211A further augmented the stress-induced increase in ASNS, CAT1, and xCT levels (Fig 6A–D). Notably, depletion of ATF4 … does cool whip have to be frozenWeb6 Dec 2024 · However, expression of TFEB-S211A and TFE3-S321A led to a near uniform pattern of p53 expression, predominantly localized in the nuclei of HeLa cells (Figure 5C). Given our data in RAW264.7 cells suggesting that loss of TFE3 and TFEB decreases p53 half-life, we performed a complimentary cycloheximide chase experiment to test if … ezy way mini storage glendale azWeb4 Mar 2024 · The master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. … does cooper make a trailer tireWeb11 Feb 2013 · The increased ability of the TFEB-S211A mutant to associate with lysosomes when compared with TFEB-WT suggests that the binding of 14-3-3 to TFEB may mask … does cooper tires have a rebate