Top/fop flash reporter assay
WebDescription; Catalogue Number: 17-285: Brand Family: Upstate: Trade Name: Upstate; Description: TCF Reporter Plasmid Kit: Overview: FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine … Web6. apr 2024 · The TOP/FOP-FLASH assay showed that the canonical Wnt activities were reduced in HONE1 and NPC43 cells, when THY1 was over expressed (Figure 2A). ... Luciferase reporter HONE1 cells were injected into nude mice via tail vein, followed by treatment with 10 mg/kg of KX2-391 or solvent control at a frequency of twice per week …
Top/fop flash reporter assay
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WebI have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and FOP, I couldn't see a high TOP/FOP ratio. WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 …
Web因此，将数个拷贝的TCF/LEF的 DNA结合位点(AGATCAAAGGgggta，大写碱基为DNA结合序列，小写的碱基为间隔序列)克隆至含TA病毒最小启动子(Minimal TA viral promoter)的萤火虫荧光素酶报告系统载体中，构建得到TOP-Flash质粒(Millipore,MA)。本研究将TOP-Flash质粒与脂质体2000(Invitrogen ... WebTOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase mi More>>
WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame. WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from …
WebPlasmid TOP-GFP from Dr. Ramesh Shivdasani's lab contains the insert 7xTOP-GFP and is published in Cancer Res. 2012 Feb 8. This plasmid is available through Addgene. ... We inserted the 7xTCF/LEF promoter cassette from the M50 Super-TOP flash plasmid (Randall Moon) into the pRRLSIN.cPPT.PGK-GFP.WPRE vector (Didier Trono), replacing the PGK ...
Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … red lion metheringhamrichard martel ohioWebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ... richard marson twitterWebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or … richard marsh town manager michiganWebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … red lion mexicaliWeb1. mar 2024 · The TOP/FOP-Flash reporter activity was presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. All experiments were performed in … red lion michiganWebReporter Assays State-of-the-art bioluminescent assays. Detect firefly, Renilla, NanoLuc® and click-beetle luciferase activities. Transfection Reagents Proven, well-cited … richard martens obituary